ribofect cp transfection kit (166t) (Ribobio co)
90
Structured Review
Ribobio co
ribofect cp transfection kit (166t)
Ribofect Cp Transfection Kit (166t), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ribofect+cp+transfection+kit/ribofect+cp+transfection+kit/pmc12198005-45-17-22
Average 90 stars, based on 1 article reviews
Ribofect Cp Transfection Kit (166t), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ribofect+cp+transfection+kit/ribofect+cp+transfection+kit/pmc12198005-45-17-22
Average 90 stars, based on 1 article reviews
ribofect cp transfection kit (166t) - by Bioz Stars,
2026-10
90/100 stars
Images
1) Product Images from "AntagomiR-192-5p-engineered exosomes encapsulated in MXene-modified GelMA hydrogel facilitated epithelization of burn wounds by targeting OLFM4"
Article Title: AntagomiR-192-5p-engineered exosomes encapsulated in MXene-modified GelMA hydrogel facilitated epithelization of burn wounds by targeting OLFM4
Journal: Bioactive Materials
doi: 10.1016/j.bioactmat.2025.06.013
Figure Legend Snippet: Overexpression of OLFM4 protected HaCaTs from dysfunction induced by miR-192-5p overexpression. A-C. RT-qPCR and immunofluorescence validation of the transfection efficiency of the OLFM4 overexpression plasmid (scale bar: 50 μm, n = 3). D. CCK8 assay to assess the effect of miR-192-5p and OLFM4 overexpression on HaCaTs cell viability (n = 3). E and F. Scratch assay to evaluate the effect of miR-192-5p and OLFM4 overexpression on HaCaTs migration ability (scale bar: 500 μm, n = 3). G and H. Flow cytometry analysis of the effect of miR-192-5p and OLFM4 overexpression on HaCaTs apoptosis (n = 3). I. ROS staining fluorescence images after overexpressing miR-192-5p and OLFM4(scale bar: 50 μm). J-K. Flow cytometry assessment of ROS levels in HaCaTs after overexpressing miR-192-5p and OLFM4 (n = 3). Data are shown as the mean ± SD. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.0001, and ns, no significance, by two-tailed unpaired Student's t -test (A and C), and by one-way ANOVA followed by the Tukey-Kramer's post hoc test (D, F, H and K).
Techniques Used: Over Expression, Quantitative RT-PCR, Immunofluorescence, Biomarker Discovery, Transfection, Plasmid Preparation, CCK-8 Assay, Wound Healing Assay, Migration, Flow Cytometry, Staining, Fluorescence, Two Tailed Test
Figure Legend Snippet: Knockdown of OLFM4 reversed the protective effect of miR-192-5p knockdown on HaCaTs function under oxidative stress model. A-C. RT-qPCR and immunofluorescence validation of the transfection efficiency of OLFM4 small interfering RNA (siRNA) (scale bar: 50 μm, n = 3). D. CCK8 assay to assess the effect of miR-192-5p and OLFM4 knockdown on HaCaTs cell viability (n = 3). E and F. Scratch assay to evaluate the effect of miR-192-5p and OLFM4 knockdown on HaCaTs migration ability (scale bar: 500 μm, n = 3). G and H. Flow cytometry analysis of the effect of miR-192-5p and OLFM4 knockdown on HaCaTs apoptosis (n = 3). I. ROS staining fluorescence images after miR-192-5p and OLFM4 knockdown (scale bar: 50 μm). J-K. Flow cytometry assessment of ROS levels in HaCaTs after knockdown of miR-192-5p and OLFM4 (n = 3). Data are shown as the mean ± SD. ∗P < 0.05, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, and ns, no significance, by two-tailed unpaired Student's t -test (A and C), and by one-way ANOVA followed by the Tukey-Kramer's post hoc test (D, F, H and K).
Techniques Used: Knockdown, Quantitative RT-PCR, Immunofluorescence, Biomarker Discovery, Transfection, Small Interfering RNA, CCK-8 Assay, Wound Healing Assay, Migration, Flow Cytometry, Staining, Fluorescence, Two Tailed Test
Figure Legend Snippet: Preparation and characterization of miR-192-5p engineered exosomes. A. TEM images of Exo-ant-192 and MSC-derived exosomes (scale bar: 100 nm). B. Particle size distribution of Exo-ant-192 and MSC-derived exosomes. C. Expression of exosome protein markers (CD81, HSP70, and TSG101) and an intracellular marker (Calnexin). D. Internalization of Exo-ant-192 and MSC-derived exosomes by HaCaTs (scale bar: 50 μm). E. Loading efficiency of Exo-Cy3-ant-192 (n = 3). F. RT-qPCR detection of intracellular miR-192-5p expression changes after transfection with Exo-ant-192 (n = 3). G. CCK8 assay to evaluate the effect of Exo-ant-192 on HaCaTs cell viability (n = 3). H and I. Effect of Exo-ant-192 on HaCaTs migration ability (scale bar: 500 μm, n = 3). J and K. Flow cytometry assessment of the effect of Exo-ant-192 on HaCaTs apoptosis (n = 3). Data are shown as the mean ± SD. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, by two-tailed unpaired Student's t -test (E, F, G, I and K).
Techniques Used: Derivative Assay, Expressing, Marker, Quantitative RT-PCR, Transfection, CCK-8 Assay, Migration, Flow Cytometry, Two Tailed Test
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